Srimad Bhagavatam Canto 04, Chapter 28, Text 46

SB 4.28.46

yada nopalabhetanghrav
 usmanam patyur arcati
asit samvigna-hrdaya
 yutha-bhrasta mrgi yatha
 
Translation by His Divine Grace A. C. Bhaktivedanta Swami Srila Prabhupada: 
 
While she was serving her husband by massaging his legs, she could feel that his feet were no longer warm and could thus understand that he had already passed from the body. She felt great anxiety upon being left alone. Bereft of her husband’s company, she felt exactly as the deer feels upon being separated from its mate.
 
Purport by His Divine Grace A. C. Bhaktivedanta Swami Srila Prabhupada: 
 
As soon as the circulation of blood and air within the body stops, it is to be understood that the soul within the body has left. The stoppage of the blood’s circulation is perceived when the hands and feet lose heat. One tests whether a body is alive or not by feeling the heart’s palpitations and the coldness of the feet and hands.
Srimad Bhagavatam Canto 04, Chapter 28, Text 45
Srimad Bhagavatam Canto 04, Chapter 28, Text 47